README for Figure 4A.csv 
*** This file contains the raw data obtained on DNA and DNA-sRok complexes using tethered particle motion represented
in Figure 4A of 
Article: The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues
Authors: Erkelens, Qin, van Erp, Miguel-Arribas, Abia, Keek, Markus, Cajili, Schwab, Meijer and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl; wmeijer@cbm.csic.es

Legend Figure 4: The bridging activity of sRok can be modulated by salt concentration A) RMS values obtained for 
32%GC DNA as a function of sRok concentration as measured by TPM in the presence of 50 mM KCl.  The RMS values 
were determined from fitting with a Gaussian distribution. Error bars represent the propagated standard deviation 
from at least two independent measurements.  Some error bars are hidden behind the data points. B) DNA recovery 
(%) as function of (s)Rok concentration in the presence of 50 mM KCl at 25°C. For reference, Rok WT is shown 
(reproduced from figure 2A). Data are plotted as mean values from three independent measurements and the error 
bars represent the standard deviation. Dashed lines are lines to guide the eye. C) Structural predictions using 
Alphafold of  Rok homodimer, sRok homodimer and Rok:sRok heterodimer (left to right). The protein structures are 
colored by the predicted lDDT-Cα (PLDDT) values with the following color scheme: blue (> 90), light blue (90 to 
70), yellow (70 to 50), orange (< 50). The PLDDT indicates the local confidence in the predicted structures from 
0-100, with 100 corresponding to highest confidence. D) SDS-PAGE analysis of His-tag pull down assay. Rok 6xhis 
was captured on HisPur™ Ni-NTA Magnetic beads and, when applicable, sRok was added in 1:1 molar ratio. E) DNA 
recovery (%) measured in the presence of 50 mM KCl + 1 mM MgCl2 at 25°C with different ratios Rok:sRok. The total 
amount of protein used was constant at 0.5 µM. F) DNA recovery (%) measured in the presence of 1 mM MgCl2 at 25°C 
and 0.5 µM protein with different KCl concentrations. 

 *** The data were obtained using Tethered particle motion as described in the associated article. 

*** Data obtained for each protein concentration(s) is given in this table as plotted in figure 4A.
Column A: sRok(nM) = Concentration of sRok in nanomolar
Column B: Replicate = number of replicate
Column C: RMS (nm) = Root mean square deviation in nanometer measured for each bead 
Column D: rho = anisotropic ratio (movement in x-direction/movement in y-direction) 
Column E: stdev = standard deviation 
Column F: FoV = field of view (data with the same FoV were collected in one image) 
Column G: bead = beads in each FoV are represented by a unique number 
The values given in column D and E are tether quality criteria and used for data filtering as described in the associated article.